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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. In radioimmunassay, a fixed concentration of labeled antigen is incubated with a constant amount of antiserum such that the concentration of antigen binding sites on the antibody is limiting. If unlabeled antigen is added to this system, there is competition between labeled antigen and unlabeled antigen for the limited and constant number of binding sites on the antibody, and thus the amount of labeled antigen bound to antibody will decrease as the concentration of unlabeled antigen increases.
Which of the following choices is correct when describing the principles of competitive radioimmunoassay procedures?

A) antibody will react preferentially with the labeled antigen
B) antibody will react equally with labeled and unlabeled antigen
C) antibody will react directly with radioactive labeled immunoglobulin
D) antibody will react with labeled antigen only
E) antibody will react preferentially with the unlabeled antigen

2. Blood Bank; Immunology
Which of the following best describes the primary function of antibodies:

A) Fix complement
B) Protect B-lymphocytes
C) Bind with antigen
D) Stimulate the immune response

3. In order to perform a venipuncture on a newly admitted hospital patient, a phlebotomist needs to

A) Realize that an admitted hospital patient has given implied consent to routine procedures such as phlebotomy.
B) Verify with the patient's primary care provider that phlebotomy is covered as a routine procedure.
C) Ask for the patients' written permission to perform the procedure.
D) Verfity that the patient has specifically name the drawing of blood in the admission process.

4. The structures involved in the production of semen include the prostate, the seminal vesicles, and the bulbourethral gland, along with the testes and epididymis.
Semen is produced as a combination of secretions from the different regions of the male reproductive tract.
Each fraction differs in chemical composition and function.
Spermatozoa are produced in the testes. They mature in the epididymis. The testes also produce testosterone and inhibin.
Fluid from the seminal vesicles accounts for approximately 70% of semen volume. The seminal vesicles are the source of fructose in semen. Fructose is used by the spermatozoa as an energy source.
The prostate gland supplies about 20% of the volume of semen. Its fluids include acid phosphatase and proteolytic enzymes that lead to coagulation and subsequent liquefaction of semen. The prostate also contains most of the IgA found in semen.
The bulbourethral gland produces mucoproteins that make up about 5% of the volume of semen.
The pituitary gland is not directly involved in the production of semen; instead hormones are released which stimulate the production of sperm. The urethra is not involved in the production of semen.
Which of the following are directly involved in the production of semen?

A) Seminal vesicles
B) Urethra
C) Pituitary gland
D) Bulbourethral gland
E) Prostate

5. The steps in the PCR process are:
1. Denaturation (Turning double stranded DNA into single strands.)
2. Annealing/Hybrization (Attachment of primers to the single DNA strands.)
3. Extension (Creating the complementary strand to produce new double stranded DNA.) What is the first step of the PCR reaction?

A) Hybridization
B) Denaturation
C) Extension
D) Annealing

Solutions: