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American Society of Microbiology ABMM Sample Questions:

1. Transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease (CJD) in humans and Bovine Spongiform Encephalopathy (BSE) in cattle, are caused by infectious agents composed primarily of:

A) Aberrantly folded host-encoded prion proteins (PrP<sup>Sc</sup>)
B) Defective viral particles requiring a helper virus
C) Viroids (small, circular RNA molecules)
D) Slow-growing bacteria lacking a cell wall

2. During the investigation of a foodborne illness outbreak linked to raw sprouts, several bacterial isolates are obtained from patient stool samples and the implicated food source. Which of the following molecular methods would be MOST useful for establishing a definitive link between the clinical isolates and the food isolate?

A) Gram staining and biochemical profiling
B) Antimicrobial susceptibility testing
C) Pulsed-field gel electrophoresis (PFGE)
D) Serotyping based on O and H antigens

3. A patient with a history of travel to Southeast Asia presents with fever, severe headache, and altered mental status. CSF analysis reveals lymphocytic pleocytosis and elevated protein, but routine bacterial and viral cultures are negative. Serological testing of CSF is positive for IgM antibodies against Japanese encephalitis virus. The MOST likely mode of transmission for this infection is:

A) Ingestion of contaminated food or water.
B) Bite of an infected mosquito.
C) Direct contact with infected animals.
D) Respiratory droplets from an infected individual.

4. A clinical microbiology laboratory is using isothermal nucleic acid amplification techniques for rapid detection of Mycobacterium tuberculosis in sputum. Compared to conventional PCR, isothermal methods offer the advantage of:

A) The ability to simultaneously amplify multiple targets in a single reaction.
B) Higher sensitivity and specificity for detecting low levels of target DNA.
C) Easier integration with downstream analysis techniques such as Sanger sequencing.
D) Faster reaction times and the ability to be performed without sophisticated thermocyclers.

5. A clinical microbiology laboratory is implementing a new multiplex nucleic acid amplification test (NAAT) for the detection of respiratory viral pathogens. To ensure the quantitative accuracy of the assay, it is essential to include:

A) External quality assessment samples.
B) Calibration standards with known concentrations of target nucleic acids.
C) Internal controls to monitor for PCR inhibition.
D) Only positive and negative controls.

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